Endotoxin-free Plasmid DNA Purification

Catalog #: EG-32
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Product Description
Description Purification of ultra-low endotoxin (endotoxin-free) grade plasmid DNA from E. coli cultures. Endotoxin-free plasmid DNA is essential for sensitive applications such as transfection into primary cells, gene therapy research, and molecular diagnostics, as endotoxins (lipopolysaccharides) can interfere with downstream experiments[1][3][6].
Yield
  • Maxiprep: 500 µg – 1 mg endotoxin-free plasmid DNA
  • Megaprep: 2.5 – 5 mg endotoxin-free plasmid DNA
  • Gigaprep: 5 – 10 mg endotoxin-free plasmid DNA
  • DNA-100: 100 mg endotoxin-free plasmid DNA
  • DNA-1000: 1000 mg endotoxin-free plasmid DNA
Quality
  • 260/280 ratio: 1.80–2.00
  • Endotoxin level: <5 EU/mg DNA
  • DNA concentration: 0.5–1 mg/mL
Customer Provides
  • Bacterial strain on agar plate or as glycerol stock
  • Pelleted cells
  • Plasmid DNA for transformation
Turnaround Time 1 to 2 weeks
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