Product Description
| Product Name | Tn5 Transposase |
| Size | 100 µg |
| Description | Tn5 Transposase is a hyperactive, recombinant enzyme derived from the Tn5 transposon of Escherichia coli. It catalyzes the efficient, sequence-independent integration (“tagmentation”) of double-stranded DNA by simultaneously fragmenting and tagging DNA with sequencing adapters. Tn5 is widely used in next-generation sequencing (NGS) library preparation workflows, including ATAC-seq, ChIPmentation, and other applications where rapid and uniform DNA fragmentation and tagging are required. |
| Applications |
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| Source | Recombinant protein expressed in E. coli |
| Fusion Tag | N-terminal 6XHis tag |
| Purification Method | Affinity chromatography (Ni-NTA) and FPLC |
| Purity | >95% as determined by SDS-PAGE |
| Storage Buffer | 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol |
| Storage | -20 °C to -80 °C (long-term); avoid repeated freeze-thaw cycles |
| Shipping | Shipped on ice packs (4 °C) or dry ice |