Product Description
| Description | Vaccinia virus Topoisomerase I is a type I eukaryotic topoisomerase that removes both positive and negative superhelical turns (right- and left-handed supercoils) from covalently closed DNA. The enzyme relaxes supercoiled DNA, resulting in covalently closed, circular DNA with reduced superhelicity. Unlike many topoisomerases, Vaccinia virus Topoisomerase I does not absolutely require Mg2+ for activity, although low concentrations of Mg2+ may enhance its function. |
| Applications |
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| Source | Recombinant protein expressed in E. coli carrying the Vaccinia virus H6R gene |
| Molecular Weight | ~32 kDa (monomer) |
| Unit Definition | One unit of Vaccinia virus DNA Topoisomerase I will convert 1 µg of supercoiled DNA (Form I) to relaxed closed circular DNA (Form II) in 1 hour at 37 °C under standard assay conditions. |
| Components | Vaccinia virus Topoisomerase I: 10,000 units/mL in 10 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5 (at 25 °C) |
| Quality Control | Free of detectable exo- and endodeoxyribonucleases, non-specific DNase, and RNase as confirmed by quality control assays. |
| Storage Condition | -20 °C |
| References | Shuman, S. "Vaccinia virus DNA topoisomerase I: a multifunctional enzyme." J Biol Chem. 1991; 266(30): 19162-19166. |
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