Human 8-oxoguanine DNA glycosylase hOGG1

Catalog #: EG-192
$495.00
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Product Description
Product Name Human 8-oxoguanine DNA Glycosylase (hOGG1)
Overview Human 8-oxoguanine DNA glycosylase (hOGG1) is a key DNA repair enzyme that initiates the base excision repair (BER) pathway by recognizing and excising 8-oxoguanine (8-oxoG), a major mutagenic lesion resulting from oxidative DNA damage. hOGG1 specifically removes 8-oxoG paired with cytosine, preventing G:C to T:A transversions and maintaining genomic stability. In addition to its glycosylase activity, hOGG1 also possesses AP lyase activity, cleaving the DNA backbone at the abasic site generated after base removal.
Gene/Protein Names
  • Gene: OGG1
  • UniProt: O15527
  • Other Names: hOGG1, 8-oxoguanine DNA glycosylase, OGG1 DNA glycosylase
Molecular Weight ~38 kDa (monomer)
Key Features
  • Recognizes and excises 8-oxoguanine lesions from DNA
  • Initiates base excision repair (BER) pathway
  • Exhibits both glycosylase and AP lyase activities
  • Prevents mutagenic G:C to T:A transversions
  • Essential for cellular defense against oxidative DNA damage
Applications
  • In vitro DNA repair assays
  • Oxidative DNA damage and repair studies
  • Genotoxicity and mutagenesis research
  • Functional studies of the base excision repair pathway
Source Recombinant protein expressed in E. coli
Quality Control
  • Purity >95% by SDS-PAGE
  • Validated for 8-oxoG excision and AP lyase activities
Formulation 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
Storage -80 °C (long-term); avoid repeated freeze-thaw cycles
References
  • Bruner, S.D., et al. "Structural basis for recognition and repair of the endogenous mutagen 8-oxoguanine in DNA." Nature. 2000; 403(6772): 859-866.
  • Boiteux, S., Radicella, J.P. "The human OGG1 gene: structure, functions, and its implication in the process of carcinogenesis." Arch Biochem Biophys. 2000; 377(1): 1-8.
  • UniProt: O15527
Product Documents
DatasheetEG-192 Product Datasheet
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