E. coli Inorganic Pyrophosphatase

• High yield RNA synthesis by in vitro transcription
• DNA polymerization reactions: preventing accumulation of pyrophosphate
• Removal of contaminant PPi in reagents used for SNP genotyping by methods based on the detection of pyrophosphate

• Inorganic Pyrophosphatase, 100 U/ml in storage buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0 @ 25°C
• 10 X Reaction Buffer: 200 mM Tris-HCl, 20 mM MgCl2, pH 7.2 @ 25°C

The absence of endo-, exodeoxyribonucleases, phosphatases, and ribonucleases is confirmed by appropriate quality tests.