Pfu DNA Polymerase

Catalog #: EG-1028
Size
500 Units
Catalog #: —
$95.00
Size
5,000 Units
Catalog #: —
$250.00
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Product Description
Product Name Pfu DNA Polymerase
Catalog Number EG-1028
Description Pfu DNA polymerase, a thermostable enzyme with 3’ to 5’ proofreading activity, derived from the pol gene of Pyrococcus furiosus expressed in Escherichia coli, catalyzes high-fidelity DNA synthesis at an optimal temperature of ~75°C with a low error rate.
Applications
  • High-fidelity PCR and primer-extension reactions
  • Generation of PCR products for cloning and expression
  • Blunt-end PCR cloning
  • RT-PCR for cDNA cloning and expression
  • Site-directed mutagenesis
Source Recombinant Pyrococcus furiosus pol gene expressed in Escherichia coli
Unit Definition One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 74°C under standard DNA polymerase assay conditions.
Components
  • Pfu DNA Polymerase: 5 units/µL in 20 mM Tris-HCl (pH 8.2), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.1% Nonidet P-40, 0.1% Tween 20, 50% glycerol, and enzyme stabilizers
  • 10X Reaction Buffer: 200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM (NH4)2SO4, 100 mM KCl, 1% Triton X-100, 1 mg/mL BSA, 20 mM MgSO4
Storage -20°C
Shipping Dry ice
Product Documents
DatasheetEG-1028 Product Datasheet
Download PDF
Published References
  1. Kim SW, Kim DU, Kim JK, Kang LW, Cho HS. “Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus.” International Journal of Biological Macromolecules 42(4):356-361 (2008). [PubMed] [DOI]
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