Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre), Endotoxin-free
Catalog #: EG-1021
$0.00
Product Description
| Description |
Recombinant Cre recombinase (TAT-Cre) was purified from an HEK293 cell line expressing enhanced form of Cre Recombinase from bacteriophage P1. This Cre recombinase has an N-terminal 6XHis tag, a Tat peptide (GRKKRRQRRRPPAGTSVSL) and an NLS sequence (PKKKRKV). Tat-Cre is the most effective protein in transduction (in vivo) and subsequent recombination compared to other forms of Cre recombinases, e.g., HNC, TCH6, HC, HNCM, CH. |
| Applications |
|
| Source | HEK 293 cells |
| Activity | A standard of 100 Units is defined as the amount of Tat-Cre in 1.0 mL of tissue culture medium that is required to induce 90% GFP expression in a HEK 293T loxP reporter cell line assay. |
| Properties |
QC: HEK293T- Cre reporter cell line 80~100% recombination efficiencies.
Figure 1. QC assay of Tat-Cre. Adding 50 and 100 units of Tat-Cre to HEK 293T cells transfected with LoxP-RFP-Stop-LoxP-GFP reporter plasmid DNA. Endotoxin Levels: < 0.1 EU/ug |
| Storage | -20ºC ~ -80ºC |
| Shipping | dry ice |
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