SARS-CoV-2 Spike Glycoprotein RBD (319-541), South Africa Variant, His tag

Catalog #: EG-323
Size
100 µg
Catalog #: —
$298.00
Size
1 mg
Catalog #: —
$2500.00
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Product Description
Product Name SARS-CoV-2 Spike Glycoprotein RBD, South African Variant, His Tag
Catalog Number EG-323
Description Recombinant SARS-CoV-2 spike glycoprotein receptor-binding domain (RBD, Arg319–Phe541, South African variant, B.1.351) with mutations K417N, E484K, and N501Y compared to Wuhan-Hu-1 (GenBank: QHD43416.1). Expressed in HEK293 cells, purified by FPLC using nickel affinity and ion exchange chromatography, with a C-terminal 6xHis tag.
Mutations K417N, E484K, N501Y
Tags C-terminal 6xHis
Purification FPLC (nickel affinity and ion exchange chromatography)
Sequence 
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFK 60
CYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGNIADYNYKLPDDFTGCVIAWNS 120
NNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQ 180
PTYGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFHHHHHH 228
Molecular Weight 26.0 kDa (theoretical)
Source HEK293 cells
Purity ≥90% by SDS-PAGE
Applications Western blot, ELISA, animal immunization studies
Storage Buffer PBS with 50% glycerol
Storage -20°C to -80°C
Shipping Dry ice
Product Documents
DatasheetEG-323 Product Datasheet
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