SARS-CoV-2 Spike Glycoprotein RBD (319-541), South Africa Variant, His tag
Catalog #: EG-323
$0.00
Product Description
| Product Name | SARS-CoV-2 Spike Glycoprotein RBD, South African Variant, His Tag |
| Catalog Number | EG-323 |
| Description | Recombinant SARS-CoV-2 spike glycoprotein receptor-binding domain (RBD, Arg319–Phe541, South African variant, B.1.351) with mutations K417N, E484K, and N501Y compared to Wuhan-Hu-1 (GenBank: QHD43416.1). Expressed in HEK293 cells, purified by FPLC using nickel affinity and ion exchange chromatography, with a C-terminal 6xHis tag. |
| Mutations | K417N, E484K, N501Y |
| Tags | C-terminal 6xHis |
| Purification | FPLC (nickel affinity and ion exchange chromatography) |
| Sequence |
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFK 60
CYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGNIADYNYKLPDDFTGCVIAWNS 120
NNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQ 180
PTYGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFHHHHHH 228
|
| Molecular Weight | 26.0 kDa (theoretical) |
| Source | HEK293 cells |
| Purity | ≥90% by SDS-PAGE |
| Applications | Western blot, ELISA, animal immunization studies |
| Storage Buffer | PBS with 50% glycerol |
| Storage | -20°C to -80°C |
| Shipping | Dry ice |
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