Product Description
| Product Name | RNase-Resistant SARS-CoV-2 mRNA qPCR Control |
| Catalog Number | EG-368 |
| Description | RNase-resistant SARS-CoV-2 mRNA is a stable mRNA-MS2 coat protein nanoparticle complex, produced in Escherichia coli via IPTG-induced expression of a plasmid encoding the MS2 coat protein and a SARS-CoV-2 mRNA sequence. The MS2 coat protein encapsidates the co-transcribed mRNA, forming pseudoviral-like particles that are DNA-free and resistant to RNase degradation. Stable at 4°C or room temperature for up to 6 months. |
| Sequence |
AUAAUGGACCCCAAAAUCAGCGAAAUGCACCCCGCAUUACGUUUGGUGGACCCUC 50 AGAUUCAACUGGCAGUAACCAGAAUGGAGAAGAACUGAUUACAAACAUUGGCCGC 100 AAAUUGCACAAUUUGCCCCCAGCGCUUCAGCGUUCUUCGGAAUGUCGCGCAUUGG 150 CGUUGCAACUGAGGGAGCCUUGAAUACACCAAAAGAUCACAUUGGCACCCGCAAU 200 CCUGCUAACAAUGCUGCAAUCGUGCUACAACUUCCUCAGUGUUUGCAGAUUUGGA 250 CCUGCGAGCGGGUUCUGACCUGAAGGCUCUGCGCGGACUUGUGGAGACAGCCGCU 300 CACCUUGGCUAUUGAGUGAAAUGGUCAUGUGUGGCGGUUCACUAUAUGUUAAACC 350 AGGUGGAACCUCAUCAGGAGAUGCCACAACUGCUUAUGCUAAUAGUGUUUUUAAC 400 AUUUGUCAAGCUGAAGAGACAGGUACGUUAAUAGUUAAUAGCGUACUUCUUUUUC 450 UUGCUUUCGUGGUAUUCUUGCUAGUUACACUAGCCAUCCUUACUGCGCUUCGAUU 500 GUGUGCGUACUGCUGCAAUAUUGUUAACGUGAG 533 |
| Titer | 1010 copies/µL |
| Size | 100 µL (1 × 1012 copies) |
| Format | Liquid |
| Quality Control | qPCR, agarose gel electrophoresis |
| Applications | Quantitative RNA standard for RT-qPCR assays |
| Storage | -80°C |
| Stability | 2 years at -80°C; up to 6 months at -40°C to 4°C |
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