E. coli Poly(A) Polymerase

Poly(A) Polymerase uses ATP as a substrate for template-independent addition of AMP from ATP to the 3´ hydroxyl termini of RNA molecules.

• Addition of a poly(A) tail to RNA synthesized in vitro for increasing RNA stability and translation efficiency prior to transferring into eukaryotic cells

• cDNA library construction using a primer containing poly(dT) on its 3´ end.

• Synthesis of polyadenylated RNA for nucleic acid amplification

• Poly(A) Polymerase: 5,000 U/ml in 20 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100, pH 7.5 @ 25°C
• 10X Reaction Buffer: 500 mM Tris-HCl, 2.5 M NaCl, 100 mM MgCl2, pH 7.9 @ 25°C

-20 °C