Exonuclease Deficient T4 DNA Polymerase

T4 DNA Polymerase catalyzes the extension of a primed DNA template (in the presence of template and primer) in the 5′→ 3′ direction. This D219A mutant T4 DNA Polymerase does not possess either 3'->5’ (proofreading) and 5′→ 3′ exonuclease activities.

Random mutagenesis

• Exonuclease Deficient T4 DNA Polymerase: 3,000 U/ml in 100 mM KPO₄, 1.0 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 6.5 @ 25°C
• 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl₂, 10 mM DTT, pH 7.9 @ 25°C

-20 °C