Product Description
| Product Name | Inorganic Pyrophosphatase (E. coli, Recombinant) |
| Catalog Number | EG-193 |
| Description |
Recombinant inorganic pyrophosphatase from Escherichia coli, expressed in E. coli and purified to high homogeneity. This enzyme catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate (Pi), a critical reaction for driving biosynthetic processes and molecular biology workflows. The enzyme is supplied in a buffered solution and is free of detectable exonuclease and endonuclease activities. |
| Synonyms | Pyrophosphate phosphohydrolase, PPase, ppa, b4226, JW4185 |
| Tag | N-terminal 6xHis (if applicable) |
| Expression System | E. coli |
| Molecular Weight | ~20–22 kDa (monomer); native enzyme is a homohexamer (~120 kDa)[1][7] |
| Purity | ≥95% by SDS-PAGE[5][7] |
| Specific Activity | ≥3500 U/mg (typical)[5][6] |
| Cofactor | Mg2+ required for activity[1][5][6] |
| Buffer/Formulation | 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 10% glycerol, 50 mM NaCl[5][7] |
| Applications |
|
| Unit Definition | One unit liberates 1 µmol of phosphate per minute from inorganic pyrophosphate at 37°C, pH 8.5[5][6] |
| Storage | -20°C (long term) |
| Shipping | Dry ice |
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