E. coli Single-Stranded DNA Binding Protein

Catalog #: EG-156
$495.00
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Product Description
Product Name E. coli Single-Stranded DNA-Binding Protein (SSB)
Overview The single-stranded DNA-binding protein (SSB) from Escherichia coli is a highly conserved, essential protein involved in all aspects of DNA metabolism, including replication, recombination, and repair. E. coli SSB functions as a homotetramer, with each subunit (~18.9 kDa) contributing to high-affinity, cooperative binding to single-stranded DNA (ssDNA). SSB protects ssDNA from nucleolytic degradation, prevents secondary structure formation, and facilitates the recruitment and activity of numerous DNA-processing enzymes. It is a prototypical model for studying protein–nucleic acid interactions and is widely used in molecular biology to enhance DNA amplification and manipulation reactions[1][2][3][4][5][6][7][8].
Size 100 µg
Concentration 1 mg/mL
Molecular Weight ~75.6 kDa (homotetramer; 18.9 kDa per subunit)
Key Features
  • Homotetrameric structure, each subunit binds ssDNA with high affinity
  • Prevents reannealing and secondary structure formation in ssDNA
  • Protects ssDNA from nuclease degradation
  • Facilitates DNA replication, repair, and recombination
  • Interacts with multiple DNA-processing enzymes via its conserved C-terminal tail
Applications
  • Enhancement of PCR, isothermal amplification, and DNA sequencing reactions
  • In vitro DNA replication and repair assays
  • Stabilization of ssDNA in molecular biology workflows
  • Model system for studying protein–nucleic acid interactions
Source Recombinant protein expressed in E. coli
Quality Control
  • Purity >99% by SDS-PAGE[4]
  • Functional validation in ssDNA-binding assays
  • Negligible exonuclease and endonuclease contamination
Formulation 50 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5
Storage -20 °C (long-term); avoid repeated freeze-thaw cycles
References
  • Meyer, R.R., Laine, P.S. The single-stranded DNA-binding protein of Escherichia coli. Microbiol Rev. 1990; 54(4):342-380.[1][2]
  • Suksombat, S., et al. Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathways. eLife. 2015;4:e08193.[5][6][7]
  • QIAGEN. E. coli Single-Stranded DNA Binding Protein. Product Data Sheet.[4]
  • Kelman, Z., et al. Molecular insights into the prototypical single-stranded DNA-binding protein of E. coli. Crit Rev Biochem Mol Biol. 2024.[8]
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