Sulfolobus solfataricus Single-stranded DNA binding proteins

Catalog #: EG-170
$495.00
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Product Description
Product Name Sulfolobus solfataricus Single-Stranded DNA-Binding Protein (SsoSSB)
Overview The single-stranded DNA-binding protein from Sulfolobus solfataricus (SsoSSB) is a thermostable protein that binds with high affinity and specificity to single-stranded DNA (ssDNA). SsoSSB plays a critical role in DNA replication, recombination, and repair in hyperthermophilic archaea. It protects ssDNA from nucleolytic degradation, prevents secondary structure formation, and facilitates the action of DNA polymerases and helicases at elevated temperatures. SsoSSB is widely used in molecular biology applications requiring robust ssDNA stabilization under extreme conditions.
Size 100 µg
Concentration 1 mg/mL
Molecular Weight ~16.3 kDa (monomer)
Key Features
  • Thermostable: active at temperatures up to 80–90 °C
  • Binds ssDNA with high affinity and specificity
  • Prevents formation of secondary structures in ssDNA
  • Facilitates DNA replication, repair, and recombination in high-temperature environments
  • Functions as a homotetramer, similar to bacterial SSBs
Applications
  • Thermostable DNA amplification (PCR, isothermal amplification)
  • In vitro DNA replication and repair assays at high temperature
  • Stabilization of ssDNA in molecular biology and biotechnology workflows
  • Model system for studying DNA-protein interactions in archaea
Source Recombinant protein expressed in E. coli
Quality Control
  • Purity >95% by SDS-PAGE
  • Functional validation in ssDNA-binding assays
Formulation 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
Storage -80 °C (long-term); avoid repeated freeze-thaw cycles
References
  • Robbins, J.B., et al. Structural and thermodynamic studies of the ssDNA-binding protein from Sulfolobus solfataricus. J Mol Biol. 2004; 340(4): 647–658.
  • Kelly, T.J., et al. Archaeal SSBs: Structure, function and applications. Extremophiles. 2010; 14(1): 1–8.
  • Richard, D.J., et al. Single-stranded DNA binding proteins from Sulfolobus solfataricus and Sulfolobus acidocaldarius. J Bacteriol. 2001; 183(19): 6075–6083.
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