FastÄQ<sup>TM</sup> Taq DNA Polymerase

Catalog #: EG-1023
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Product Description
Description FastÄQTM Taq DNA polymerase is an engineered Taq to achieve rapid PCR without compromising specificity and accuracy. It can extend a kilobase of sequence in 15 seconds and with accuracy 50 times higher than Taq DNA Polymerasey. This ultra-thermostable polymerase can be used for amplifying sequencing libraries.
Source A recombinant E. coli strain carrying the engineered FastÄQTM Taq DNA polymerase gene.
Applications
  • Routine PCR amplification of up to 20 kb DNA fragment.
  • Blunt-end PCR cloning.
  • Sequencing library preparation
Unit Definition One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble form at 74°C in 30 minutes.
Features
  • High yield, high sensitivity, ultrafast FastÄQTaq DNA Polymerase
  • Purity: >99%
  • Specific Activity: >10,000 U/mg
  • Stable at 4°C
Components
  • FastÄQTM Taq DNA polymerase - 5 units/µL in 50% glycerol, 20 mM Tris-HCl, pH 7.4, 40 mM NaCl,100 mM KCl, 0.1 mM EDTA, and enzyme stabilizers.
  • 10X Reaction Buffer: 160 mM (NH4)2SO4 , 670 mM Tris-HCl, pH 8.8 (at 25 °C), 15 mM MgCl2 , 0.1% Tween 20
Storage FastÄQTM Taq DNA polymerase is stable for 2 years when stored in a constant temperature freezer at -20°C.
Shipping 4°C
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