3C HiFi DNA Polymerase

Catalog #: EG-1027

Size	Catalog #	Price	Add to Cart
250 Units	—	$188.00
1,000 Units	—	$680.00
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Size

250 Units

Catalog #: —

$188.00

Size

1,000 Units

Catalog #: —

$680.00

Product Description

Product Name	3C HiFi DNA Polymerase
Catalog Number	EG-1027
Description	3C HiFi DNA Polymerase, a recombinant thermostable enzyme with 3’ to 5’ proofreading activity, derived from an engineered Thermococcus-like DNA polymerase gene expressed in Escherichia coli, amplifies DNA up to 20 kb with processivity and elongation rates at least fivefold higher than Pfu DNA polymerase, optimized for high-fidelity PCR at ~72°C.
Applications	High-fidelity PCR amplification Blunt-end PCR cloning
Source	Recombinant Escherichia coli expressing an engineered Thermococcus-like DNA polymerase gene
Unit Definition	One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C under standard DNA polymerase assay conditions.
Components	3C HiFi DNA Polymerase: 5 units/µL in 20 mM Tris-HCl (pH 8.0), 40 mM NaCl, 2 mM sodium phosphate, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol, and stabilizers 10X Reaction Buffer: 200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM (NH₄)₂SO₄, 100 mM KCl, 1% Triton X-100, 1 mg/mL BSA, 20 mM MgSO₄
Storage	-20°C
Shipping	Dry ice

Product Documents

DatasheetEG-1027 Product Datasheet

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Catalog #: EG-1018$198.00

HiFi ÄQStart™ Taq Mastermix

Catalog #: EG-1017$168.00