3C HiFi DNA Polymerase

Catalog #: EG-1027
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Product Description
Product Name 3C HiFi DNA Polymerase
Catalog Number EG-1027
Description 3C HiFi DNA Polymerase, a recombinant thermostable enzyme with 3’ to 5’ proofreading activity, derived from an engineered Thermococcus-like DNA polymerase gene expressed in Escherichia coli, amplifies DNA up to 20 kb with processivity and elongation rates at least fivefold higher than Pfu DNA polymerase, optimized for high-fidelity PCR at ~72°C.
Applications
  • High-fidelity PCR amplification
  • Blunt-end PCR cloning
Source Recombinant Escherichia coli expressing an engineered Thermococcus-like DNA polymerase gene
Unit Definition One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C under standard DNA polymerase assay conditions.
Components
  • 3C HiFi DNA Polymerase: 5 units/µL in 20 mM Tris-HCl (pH 8.0), 40 mM NaCl, 2 mM sodium phosphate, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol, and stabilizers
  • 10X Reaction Buffer: 200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM (NH4)2SO4, 100 mM KCl, 1% Triton X-100, 1 mg/mL BSA, 20 mM MgSO4
Storage -20°C
Shipping Dry ice
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