NLS-Cre Recombinase mRNA, Animal-free

NLS-Cre Recombinase mRNA, Animal-free

Catalog #: EG-2895
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Product Description
Product Name NLS-Cre Recombinase (NLS-Cre) mRNA
Catalog Number EG-2589
Description NLS-Cre mRNA, synthesized from animal-free components, expresses a human- and mouse-codon-optimized Cre recombinase fused to a nuclear localization sequence (NLS), enabling efficient ex vivo loxP recombination in cell lines and primary cells.
Codon Optimization Human and mouse
Modifications
  • 5’ capped with ARCA (m7(3’-O-methyl)-G(5’)ppp(5’)G, Cap 0) or CleanCap AG (m7G(5’)ppp(5’)(2’-OMeA)pG, Cap 1)
  • Pseudouridine-5’-triphosphate (Pseudo-UTP)
  • 5-Methylcytidine-5’-triphosphate (5mCTP)
  • Poly-A tail: 180 nucleotides
Applications
  • Ex vivo loxP recombination in cell lines and primary cells
Concentration ~1 µg/µL
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
Quality Control
  • Integrity verified by agarose gel electrophoresis
  • Functional activity confirmed by transfection of HEK293 cells with a loxP reporter plasmid
Storage -80°C
Shipping Dry ice
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