NLS-FLP Recombinase mRNA, Animal-Free

Catalog #: EG-1044
Size
ARCA 10 µg
Catalog #: —
$480.00
Size
ARCA 20 µg
Catalog #: —
$950.00
Size
ARCA 100 µg
Catalog #: —
$4500.00
Size
Cleancap 10 µg
Catalog #: —
$480.00
Size
Cleancap 20 µg
Catalog #: —
$980.00
Size
CleanCap 100 µg
Catalog #: —
$4500.00
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Product Description
Product Name NLS-FLP Recombinase mRNA
Description

This NLS-FLP recombinase mRNA encodes a human codon-optimized Flp recombinase fused to nuclear localization sequence, it can be used for FRT recombination in cell lines and primary cells.

mRNA is made from animal-free components.
Applications ex vivo FRT recombination
Modifications
  • 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
  • Fully substituted with Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Concentration ~1 µg/µl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC
  • Agarose gel electrophoresis
  • Activity assay: HEK293 cells transfected with FRT reporter plasmid
Shipping Dry ice
Storage -80 oC
Product Documents
DatasheetEG-1044 Product Datasheet
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