Thermus aquaticus Muts DNA mismatch repair protein, Taq-Muts, no tag

The Thermus aquaticus MutS protein is a thermostable DNA mismatch repair enzyme that specifically recognizes and binds to heteroduplex DNA containing:

• Single base mismatches (GT, CT, AG, etc.)
• Insertion/deletion loops (1-4 bases)
• Unpaired bases

Key Features:

• Broad temperature activity range (0-75°C) with optimal binding at 65°C
• Thermostable ATPase activity (k_cat = 0.5 min^-1 at 65°C)
• High binding affinity (K_d = 10-50 nM for mismatches)
• Native sequence without fusion tags
• >95% purity by SDS-PAGE analysis

• Gene synthesis quality control: Removal of mismatch DNA from PCR assemblies
• Mutation detection: Identification of SNPs and small indels
• Isothermal SNP detection: Rapid diagnostics without thermal cycling
• DNA heteroduplex analysis: Characterization of recombination products

• Molecular Weight: 95.2 kDa (calculated)
• Extinction Coefficient: 99,430 M^-1cm^-1 (280 nm)
• Purity: >95% by SDS-PAGE
• Accession #: AAC43637 (NCBI)

1  MEGMLKGEGP GPLPPLLQQY VELRDQYPDY LLLFQVGDFY ECFGEDAERL ARALGLVLTH
61 KTSKDFTTPM AGIPLRAFEA YAERLLKMGF RLAVADQVEP AEEAEGLVRR EVTQLLTPGT
121 LLQESLLPRE ANYLAAIATG DGWGLAFLDV STGEFKGTVL KSKSALYDEL FRHRPAEVLL
181 APELLENGAF LDEFRKRFPV MLSEAPFEPE GEGPLALRRA RGALLAYAQR TQGGALSLQP
241 FRFYDPGAFM RLPEATLRAL EVFEPLRGQD TLFSVLDETR TAPGRRLLQS WLRHPLLDRG
301 PLEARLDRVE GFVREGALRE GVRRLLYRLA DLERLATRLE LGRASPKDLG ALRRSLQILP
361 ELRALLGEEV GLPDLSPLKE ELEAALVEDP PLKVSEGGLI REGYDPDLDA LRAAHREGVA
421 YFLELEERER ERTGIPTLKV GYNAVFGYYL EVTRPYYERV PKEYRPVQTL KDRQRYTLPE
481 MKEKEREVYR LEALIRRREE EVFLEVRERA KRQAEALREA ARILAELDVY AALAEVAVRY
541 GYVRPRFGDR LQIRAGRHPV VERRTEFVPN DLEMAHELVL ITGPNMAGKS TFLRQTALIA
601 LLAQVGSFVP AEEAHLPLFD GIYTRIGASD DLAGGKSTFM VEMEEVALIL KEATENSLVL
661 LDEVGRGTSS LDGVAIATAV AEALHERRAY TLFATHYFEL TALGLPRLKN LHVAAREEAG
721 GLVFYHQVLP GPASKSYGVE VAAMAGLPKE VVARARALLQ AMAARREGAL DAVLERLLAL
781 DPDRLTPLEA LRLLQELKAL ALGAPLDTMK G
        

Storage Buffer:
20 mM Tris-HCl (pH 8.0), 250 mM NaCl, 1 mM DTT, 50% glycerol

10× Reaction Buffer:
100 mM KCl, 50 mM Tris-HCl (pH 8.5), 10 mM MgCl₂, 0.1 mM EDTA, 1 mM DTT, 2% glycerol

• Long-term: -80°C (stable for 24 months)
• Short-term: -20°C (stable for 6 months)
• Shipping: Dry ice
• Note: Aliquot to avoid repeated freeze-thaw cycles

1. Carr PA, et al. (2004) Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res 32(20):e162. doi:10.1093/nar/gnh160
2. Matzas M, et al. (2005) Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Res 33(6):e55. doi:10.1093/nar/gni054
3. Bui CT, et al. (2007) Rapid SNP diagnostics using asymmetric isothermal amplification. Nat Methods 4:257-262. doi:10.1038/nmeth1007