NLS-Cas9-D10A Nickase mRNA

NLS-Cas9-D10A Nickase mRNA encodes a Streptococcus pyogenes SF370 Cas9 protein D10A mutant with nuclear localization signal (NLS) at C terminus. Unlike the wild-type CAS9, which cleaves the double strand DNA generating two break sites based on its two active domains, the NLS-Cas9-D10A Nickase has only one active domain, thus it can only cut one single strand DNA that is complementary to the guide-RNA, producing one single strand break. This mutant mRNA favors homology-directed repair and decreases the occurrence of non-homologous end joining.

• 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
• Pseudo-UTP
• 5mCTP
• PolyA: 180 nt