EGFP Self-Amplifying mRNA

Catalog #: EG-320
Product Description
Product Name EGFP Self-Amplifying mRNA
Description This self-amplifying mRNA encodes the enhanced green fluorescent protein (EGFP) along with four nonstructural proteins (nsP1–nsP4) derived from the positive-sense single-stranded RNA alphavirus Venezuelan equine encephalitis virus (VEEV). These nonstructural proteins mediate intracellular RNA replication, enabling prolonged and enhanced transgene expression.
Modifications
  • 5' capped using the vaccinia capping enzyme system and 2'-O-methyltransferase to generate a Cap-1 structure
  • 100% substitution with pseudouridine triphosphate (Ψ-UTP)
  • 30% substitution with 5-methylcytidine triphosphate (5mCTP)
  • Poly(A) tail: 180 nucleotides
Application Reporter assay and gene expression studies
Concentration ~1 µg/µL
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC Agarose gel electrophoresis
Shipping Dry ice
Storage -80 °C
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