Cas9 recombinant protein, NLS-Cas9 , S. pyogenes

Recombinant CRISPR-Cas9 nuclease engineered for enhanced cellular delivery and nuclear localization. This protein contains:

• N-terminal modifications:
  • TAT cell-penetrating peptide (GRKKRRQRRRPPAGTSV)
  • SV40 nuclear localization signal (KKKRKV)
• C-terminal modifications:
  • SV40 nuclear localization signal
  • 6×His tag for purification
• Source: Purified from codon-optimized E. coli expression system

• Genome editing:
  • Cell culture transfection
  • One-cell embryo microinjection
  • Organoid engineering
• In vitro applications:
  • Targeted DNA cleavage assays
  • Protospacer adjacent motif (PAM) validation
  • gRNA activity testing

• Molecular Weight: ~160 kDa (theoretical)
• Purity: >95% (SDS-PAGE with Coomassie staining)
• Storage Buffer: 10 mM HEPES (pH 7.4), 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol
• Activity: >90% target cleavage efficiency with validated gRNAs

• -80°C: 2 years
• -20°C: 1 year
• 4°C: 1 month

For genome editing in cultured cells:

1. Complex with gRNA (molar ratio 1:2) for 10 min at room temperature
2. Deliver using appropriate transfection method
3. Typical working concentration: 0.5-2 µM