Protein Expression and Purification using Bacterial Expression System

Protein Expression and Purification using Bacterial Expression System

Service Code: EG-1076
$2170.00
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Service Description
Service Name Protein Expression and Purification using Bacterial Expression System
Description Our Bacterial Expression and Purification Service offers rapid, cost-effective production of recombinant proteins in E. coli using advanced fusion tags and dual-tag vectors for enhanced expression, solubility, and streamlined purification. This platform is ideal for research, assay development, and preparative-scale protein production, delivering high-purity proteins suitable for downstream analysis or functional studies.
Key Features & Advantages
  • Enhanced expression with GST, MBP, and NusA fusion tags
  • Dual-tag vectors (His-GST, His-MBP, His-Strep) for simple and efficient purification
  • Optional fluorescent (GFP, RFP), luciferase, or detection tags (HA, Myc, Flag) for downstream assays
  • High-yield, scalable production in E. coli
  • AKTA FPLC purification for >95% purity
Workflow & Timeline
  1. Cloning (2 weeks):
    • Gene cloning or subcloning into expression vector
    • Verification by restriction digestion and sequencing
    • Plasmid DNA preparation
  2. Expression Optimization (1–2 weeks):
    • Transformation of competent cells
    • Optimization of induction (time, temperature, IPTG concentration)
    • Expression analysis by SDS-PAGE, dot-blot, or Western blot
  3. Large-Scale Expression & Purification (1–2 weeks):
    • Large-scale culture and protein expression
    • Purification using AKTA FPLC
    • Dialysis, concentration, and QC (SDS-PAGE, Western blot, protein quantitation)
    • Delivery of purified, ready-to-use protein
Deliverables
  • >3 mg purified recombinant protein (>95% purity)
  • Quality control data (SDS-PAGE, Western blot, yield)
  • Chromatograms and process documentation
Required Materials
  • 5 µg plasmid containing gene of interest (with complete vector sequence in electronic format)
  • or protein sequence for gene synthesis (additional fee applies)
  • or accession number for cDNA sourcing
Applications
  • Research and assay development
  • Structural biology and functional studies
  • Antibody production and immunization
Consultation & Support Free project consultation and technical support throughout your project.
Quotation & Ordering
  • Request a quotation form from customer service: crm@excellgen.com
  • Submit the completed form and PO/credit card info by email, fax, or phone
  • Ship samples with a hard copy of the completed form to:
    Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743
Service Documents
DatasheetEG-1076 Product Datasheet
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