IgE Purification from Culture Medium

Service Code: EG-1076-1
$750.00
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Service Description
Service Name IgE Purification from Culture Medium
Description Our IgE purification service isolates and purifies immunoglobulin E (IgE) antibodies from mammalian cell culture supernatant. Using optimized affinity chromatography and polishing steps, we deliver high-purity, functional IgE suitable for research, diagnostics, and functional assays.
Workflow
  1. Clarification of culture medium by centrifugation and filtration
  2. Affinity purification using anti-IgE or custom resin
  3. Polishing by size exclusion or ion exchange chromatography (AKTA FPLC)
  4. Buffer exchange and concentration
  5. Quality control (SDS-PAGE, Western blot, yield determination)
Advantages
  • High specificity and purity (>95%)
  • Preserves IgE structure and function
  • Scalable for small or large volumes
  • Fast turnaround and expert technical support
Deliverables
  • Pooled, purified IgE antibody (>95% purity)
  • Quality control data (SDS-PAGE, Western blot, yield)
  • Chromatograms and process documentation
Required Materials
  • Cell culture supernatant containing secreted IgE
  • Information on cell line and medium composition
  • Desired buffer and concentration (if specific requirements)
Applications
  • Allergy and immunology research
  • Diagnostic assay development
  • Functional and binding studies
Consultation & Support Free project consultation and technical support throughout your project.
Quotation & Ordering
  • Request a quotation form: crm@excellgen.com
  • Submit the completed form and PO/credit card info by email, fax, or phone
  • Ship samples with a hard copy of the completed form to:
    Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743
Service Documents
DatasheetEG-1076-1 Product Datasheet
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