Protein Production by Cell-Free Protein Synthesis

Our comprehensive Cell-Free Protein Synthesis service provides rapid, flexible production of recombinant proteins without living cells. Utilizing advanced prokaryotic and eukaryotic lysate systems, we deliver high-quality proteins for research, structural biology, and drug discovery applications. With 15+ years of protein expression expertise, we specialize in challenging targets including membrane proteins, toxic proteins, and those requiring non-natural amino acid incorporation.

• Rapid Turnaround: Protein production in 1-2 weeks (vs 4-6 weeks for cell-based systems)
• High Success Rate: Natural protein folding with >90% active proteins
• Challenging Targets: Ideal for toxic, insoluble, or membrane proteins
• Custom Modifications: Incorporation of labeled amino acids, non-natural amino acids, or stable isotopes
• Scalable: From 100 μg to milligram quantities
• Multiple Systems: E. coli, wheat germ, mammalian, and insect lysates available

• Prokaryotic: NEB PURExpress, Qiagen EasyXpress, Roche ProteoMaster E. coli
• Eukaryotic: Roche RTS Wheat Germ, CellFree Sciences ENDEXT, Thermo Scientific Human In Vitro
• Specialty: Promega TNT, Invitrogen Expressway™

• Structural biology (X-ray crystallography, NMR, cryo-EM)
• Antibody production and protein array development
• Drug discovery and high-throughput screening
• Enzyme engineering and characterization
• Incorporation of modified amino acids or stable isotopes

• Purified protein in buffer of choice (0.1-10 mg typical yield)
• Complete QC package (SDS-PAGE, Western blot, activity data)
• ÄKTA FPLC chromatograms and purification report
• Expression optimization recommendations

Submit protein sequence, accession number, or plasmid DNA to:
Protein Services, Excellgen, Inc.
15601 Crabbs Branch Way, Rockville, MD 20855-2743

For quotes: crm@excellgen.com