2 L Expression and FPLC Purification

Service Code: EG-1076-3
$1200.00
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Service Description
Service Name 2 L Expression and FPLC Purification Service
Description Our 2 L Expression and FPLC Purification Service provides high-yield recombinant protein production and purification from a 2-liter culture scale. This comprehensive service includes small-scale test expression, large-scale expression in your preferred host (typically E. coli), and purification using state-of-the-art ÄKTA FPLC (Fast Protein Liquid Chromatography) systems. The workflow is optimized for maximum protein yield, purity, and activity, and is suitable for research, assay development, and structural biology applications.
Key Features
  • 2-liter culture scale for robust protein yields
  • Customizable expression in E. coli or other hosts (inquire for yeast, insect, or mammalian options)
  • Purification via affinity, ion exchange, size exclusion, or multi-step FPLC workflows
  • Compatible with a wide range of affinity tags (His, GST, MBP, Strep, etc.)
  • Optional tag removal and polishing steps
Workflow
  1. Small-scale expression testing and optimization
  2. Large-scale (2 L) culture expression
  3. Cell harvest and lysis
  4. FPLC purification (affinity, ion exchange, size exclusion, or combination)
  5. Buffer exchange and concentration (if required)
  6. Comprehensive quality control
Quality Control
  • SDS-PAGE and Coomassie staining (>95% purity)
  • Western blot analysis (if antibody is available)
  • Protein concentration determination
  • Chromatograms and process documentation
Deliverables
  • Pooled, purified protein in your specified buffer and concentration
  • Quality control report (SDS-PAGE, purity, yield, activity, etc.)
  • Chromatograms and method summary
  • Optional: expression plasmid, cell pellets, or lysate upon request
Sample Requirements
  • Expression plasmid or gene sequence
  • Preferred tag and purification method (if applicable)
  • Any special buffer or storage requirements
Applications
  • Structural biology (X-ray, NMR, cryo-EM)
  • Enzyme assays and functional studies
  • Antibody production
  • Drug screening and target validation
Consultation & Support Free project consultation and technical support throughout your project.
Contact Contact us for a custom quote and to discuss your specific project requirements.
Service Documents
DatasheetEG-1076-3 Product Datasheet
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