| Service Name |
Fusion Tag Removal and FPLC Purification |
| Description |
Our Fusion Tag Removal and FPLC Purification service provides precise, efficient removal of affinity and detection tags from recombinant proteins, followed by high-resolution purification using AKTA FPLC systems. We utilize site-specific proteases (such as HRV 3C, TEV, or thrombin) to cleave tags at engineered recognition sites, ensuring minimal impact on protein structure and function. The workflow includes a reverse purification step to separate the cleaved tag and protease, delivering tag-free, high-purity protein for downstream applications.
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| Key Features & Advantages |
- Site-specific tag removal using HRV 3C, TEV, or thrombin protease, compatible with a wide range of fusion tags (His, GST, Strep, MBP, etc.)
- Efficient cleavage at engineered protease recognition sites (e.g., HRV 3C site)
- Reverse purification to remove cleaved tag and protease, ensuring high protein purity
- Final polishing by AKTA FPLC (affinity, ion exchange, or size exclusion as needed)
- Delivery of tag-free, functionally active protein suitable for structural, functional, or therapeutic studies
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| Workflow |
- Protease cleavage of fusion tag at engineered recognition site (e.g., HRV 3C site)[1]
- Reverse purification step to remove cleaved tag and protease[1]
- Final purification and polishing using AKTA FPLC
- Comprehensive quality control (SDS-PAGE, Western blot, activity assay if required)
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| Deliverables |
- Tag-free, purified recombinant protein (>95% purity)
- Quality control data (SDS-PAGE, Western blot, yield, activity if applicable)
- Chromatograms and process documentation
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| Required Materials |
- Purified fusion-tagged protein (with engineered protease recognition site)
- Information on tag type and cleavage site sequence
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| Applications |
- Structural biology and crystallography
- Enzyme assays and functional studies
- Therapeutic protein development
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| Consultation & Support |
Free project consultation and technical support throughout your project.
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| Quotation & Ordering |
- Contact customer service to discuss your project and receive a custom quote
- Submit required materials and project details by email or shipment
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