Fusion Tag Removal and FPLC Purification

Service Code: EG-1076-4
$1500.00
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Service Description
Service Name Fusion Tag Removal and FPLC Purification
Description Our Fusion Tag Removal and FPLC Purification service provides precise, efficient removal of affinity and detection tags from recombinant proteins, followed by high-resolution purification using AKTA FPLC systems. We utilize site-specific proteases (such as HRV 3C, TEV, or thrombin) to cleave tags at engineered recognition sites, ensuring minimal impact on protein structure and function. The workflow includes a reverse purification step to separate the cleaved tag and protease, delivering tag-free, high-purity protein for downstream applications.
Key Features & Advantages
  • Site-specific tag removal using HRV 3C, TEV, or thrombin protease, compatible with a wide range of fusion tags (His, GST, Strep, MBP, etc.)
  • Efficient cleavage at engineered protease recognition sites (e.g., HRV 3C site)
  • Reverse purification to remove cleaved tag and protease, ensuring high protein purity
  • Final polishing by AKTA FPLC (affinity, ion exchange, or size exclusion as needed)
  • Delivery of tag-free, functionally active protein suitable for structural, functional, or therapeutic studies
Workflow
  1. Protease cleavage of fusion tag at engineered recognition site (e.g., HRV 3C site)[1]
  2. Reverse purification step to remove cleaved tag and protease[1]
  3. Final purification and polishing using AKTA FPLC
  4. Comprehensive quality control (SDS-PAGE, Western blot, activity assay if required)
Deliverables
  • Tag-free, purified recombinant protein (>95% purity)
  • Quality control data (SDS-PAGE, Western blot, yield, activity if applicable)
  • Chromatograms and process documentation
Required Materials
  • Purified fusion-tagged protein (with engineered protease recognition site)
  • Information on tag type and cleavage site sequence
Applications
  • Structural biology and crystallography
  • Enzyme assays and functional studies
  • Therapeutic protein development
Consultation & Support Free project consultation and technical support throughout your project.
Quotation & Ordering
  • Contact customer service to discuss your project and receive a custom quote
  • Submit required materials and project details by email or shipment
Service Documents
DatasheetEG-1076-4 Product Datasheet
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