Cre Recombinase, HNC

Recombinant Cre recombinase HNCre was purified from an E. coli strain carrying an engineered plasmid encoding Cre Recombinase from bacteriophage P1 with additional N-terminal 6XHis tag and an NLS sequence (PKKKRKV). This Cre recombinase has lower cell permeability than Tat-Cre, but can be transported into cells using cell permeable peptides and some protein transfection reagents. It is effective in recombination in various cell types after being transported into cells

• In vitro LoxP recombination for subcloning or vector/clone engineering

• Transduction into cultured cells including stem cells ex Vivo

Molecular Weight: 40 kDa

Endotoxin Levels: < 0.1 EU/ug

Purity: >95% by SDS-PAGE and HPLC analysis