Klenow Fragment

Catalog #: EG-1055

$0.00

Product Description

Product Name	Klenow Fragment
Catalog Number	EG-1055
Description	Klenow Fragment, a truncated form of Escherichia coli DNA Polymerase I expressed recombinantly in Escherichia coli, catalyzes 5’ to 3’ DNA synthesis and 3’ to 5’ proofreading exonuclease activity but lacks 5’ to 3’ exonuclease activity, suitable for blunt-end DNA synthesis and labeling applications.
Applications	Conversion of 3’ overhangs to blunt ends Random-primed DNA labeling Fill-in of 5’ overhangs of dsDNA to form blunt ends DNA sequencing by the Sanger method Site-directed mutagenesis with synthetic oligonucleotides Second-strand cDNA synthesis
Source	Recombinant Escherichia coli expressing the Klenow Fragment of the polA gene
Unit Definition	One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard DNA polymerase assay conditions.
Components	Klenow Fragment: 5,000 units/mL in 25 mM Tris-HCl (pH 7.4 at 25°C), 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl (pH 7.9 at 25°C), 100 mM MgCl₂, 10 mM DTT
Quality Control	Absence of endodeoxyribonucleases confirmed by appropriate quality tests Functionally validated for fill-in of 5’ overhangs of dsDNA and random-primed DNA labeling
Storage	-20°C
Shipping	Dry ice

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