Klenow Fragment (3'->5' exo-)

Catalog #: EG-1056

$0.00

Product Description

Product Name	Klenow Fragment (3’ to 5’ exo-)
Catalog Number	EG-1056
Description	Klenow Fragment (3’ to 5’ exo-), a recombinant N-terminal truncation of Escherichia coli DNA Polymerase I with mutations (D355A, E357A), retains 5’ to 3’ polymerase activity but lacks 5’ to 3’ exonuclease and 3’ to 5’ proofreading exonuclease activities, ideal for DNA labeling and sequencing applications.
Applications	Random-primed DNA labeling DNA sequencing by the Sanger method Second-strand cDNA synthesis Fill-in of 5’ overhangs of dsDNA to form blunt ends Strand displacement amplification (SDA)
Source	Recombinant Escherichia coli expressing the mutated Klenow Fragment of the polA gene
Unit Definition	One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard DNA polymerase assay conditions.
Components	Klenow Fragment (3’ to 5’ exo-): 5,000 units/mL in 25 mM Tris-HCl (pH 7.4 at 25°C), 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl (pH 7.9 at 25°C), 100 mM MgCl₂, 10 mM DTT
Quality Control	Absence of endo- and exodeoxyribonucleases confirmed by appropriate quality tests Functionally validated for random-primed DNA labeling
Storage	-20°C
Shipping	Dry ice

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