phi29 (φ29) DNA Polymerase

Catalog #: EG-1057

$0.00

Product Description

Product Name	φ29 DNA Polymerase
Catalog Number	EG-1057
Description	φ29 DNA Polymerase, a highly processive enzyme from Bacillus subtilis bacteriophage φ29 expressed recombinantly in Escherichia coli, catalyzes high-fidelity 5’ to 3’ DNA synthesis with 3’ to 5’ proofreading exonuclease activity on single-stranded DNA and exceptional strand displacement, ideal for isothermal DNA amplification.
Applications	Rolling circle amplification (RCA) for generating periodic DNA nanotemplates Multiple displacement amplification (MDA) Whole-genome amplification (WGA) for: SNP and STR detection Cell-free amplification from single cells Pathogenic organisms or metagenomes Amplification from filter paper blood spot samples DNA template preparation for sequencing Protein-primed DNA amplification In situ genotyping with padlock probes Recombination-based cloning Cell-free cloning of lethal DNA RNA-primed DNA amplification
Source	Recombinant Escherichia coli expressing the φ29 DNA polymerase gene from Bacillus subtilis bacteriophage φ29
Unit Definition	One unit is defined as the amount of enzyme that incorporates 0.5 pmol of dNTPs into acid-insoluble material in 10 minutes at 30°C under standard DNA polymerase assay conditions.
Components	φ29 DNA Polymerase: 100,000 units/mL in 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Tween 20, 0.5% (v/v) Nonidet P-40, 50% (v/v) glycerol 10X Reaction Buffer: 500 mM Tris-HCl (pH 7.5 at 25°C), 100 mM (NH₄)₂SO₄, 100 mM MgCl₂, 40 mM DTT
Quality Control	Absence of endodeoxyribonucleases confirmed by appropriate quality tests Functionally validated for rolling circle amplification (RCA) and multiple displacement amplification (MDA)
Storage	-20°C
Shipping	Dry ice

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