T4 DNA Polymerase

Catalog #: EG-1058

$0.00

Product Description

Product Name	T4 DNA Polymerase
Catalog Number	EG-1058
Description	T4 DNA Polymerase, a recombinant enzyme from bacteriophage T4 expressed in Escherichia coli, catalyzes 5’ to 3’ DNA synthesis with robust 3’ to 5’ exonuclease activity but lacks 5’ to 3’ exonuclease and strand displacement activities, ideal for DNA blunting and labeling applications.
Applications	Blunting of DNA ends by fill-in of 5’ overhangs or removal of 3’ overhangs Blunting of PCR products with 3’-dA overhangs Ligation-independent cloning of PCR products via single-strand deletion subcloning Second-strand synthesis in site-directed mutagenesis Probe labeling using replacement synthesis
Source	Recombinant Escherichia coli expressing the T4 bacteriophage gene 43
Unit Definition	One unit is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard DNA polymerase assay conditions.
Components	T4 DNA Polymerase: 3,000 units/mL in 100 mM potassium phosphate (pH 6.5 at 25°C), 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl (pH 7.9 at 25°C), 100 mM MgCl₂, 10 mM DTT
Quality Control	Absence of endodeoxyribonucleases confirmed by appropriate quality tests Functionally validated for blunting of DNA ends
Storage	-20°C
Shipping	Dry ice

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