T7 DNA Polymerase

Catalog #: EG-1059

$0.00

Product Description

Product Name	T7 DNA Polymerase
Catalog Number	EG-1059
Description	T7 DNA Polymerase, a highly processive enzyme composed of an 80 kDa T7 bacteriophage gene 5 protein and a 12 kDa Escherichia coli thioredoxin (trxA gene product) expressed recombinantly in Escherichia coli, catalyzes 5’ to 3’ DNA synthesis with robust 3’ to 5’ exonuclease activity on single- and double-stranded DNA, ideal for long-template synthesis and DNA labeling.
Applications	Purification of covalently closed circular DNA by removing residual genomic DNA Primer extension reactions on long templates DNA 3’-end labeling Strand extension in site-directed mutagenesis Fill-in blunting of 5’ overhangs Second-strand cDNA synthesis In situ detection of DNA fragmentation associated with apoptosis
Source	Recombinant Escherichia coli co-expressing the T7 bacteriophage gene 5 and Escherichia coli trxA gene
Unit Definition	One unit is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard DNA polymerase assay conditions.
Components	T7 DNA Polymerase: 10,000 units/mL in 50 mM potassium phosphate (pH 7.0 at 25°C), 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol 10X Reaction Buffer: 200 mM Tris-HCl (pH 7.5 at 25°C), 100 mM MgCl₂, 10 mM DTT
Quality Control	Absence of endodeoxyribonucleases confirmed by appropriate quality tests Functionally validated for primer extension on long templates
Storage	-20°C
Shipping	Dry ice

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