Protein Expression and Purification Service

Catalog #: EG-1076-8
$3500.00
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Product Description
Service Name Protein Expression and Purification Service
Description Our Protein Expression and Purification Service provides comprehensive, customizable solutions for the production of high-quality recombinant proteins tailored to your research needs. We offer a range of expression systems—including E. coli, yeast, insect, and mammalian cells—and purification strategies to deliver proteins with high purity, yield, and activity. From gene synthesis and vector construction to scale-up and quality control, our experienced team ensures reliable, reproducible results for applications in structural biology, functional assays, drug discovery, and more.
Key Features
  • Multiple expression systems: E. coli, yeast, baculovirus-insect cells, mammalian cells
  • Customizable tags and fusion partners (e.g., His, GST, MBP, FLAG)
  • Flexible scale: from milligram to multi-gram quantities
  • Affinity, ion exchange, size exclusion, and custom purification methods
  • Endotoxin removal and tag cleavage options available
  • Comprehensive quality control: SDS-PAGE, Western blot, activity assays, mass spectrometry
Workflow
  1. Gene synthesis or subcloning
  2. Vector construction and sequence verification
  3. Small-scale expression testing and optimization
  4. Large-scale protein expression
  5. Protein purification and polishing
  6. Quality control and final reporting
Applications
  • Structural biology (X-ray, NMR, cryo-EM)
  • Enzymatic and functional assays
  • Antibody production
  • Drug screening and target validation
  • Diagnostics and industrial applications
Deliverables
  • Purified recombinant protein (custom buffer and concentration)
  • Quality control report (SDS-PAGE, purity, yield, activity, etc.)
  • Optional: expression plasmid, cell pellets, or lysate upon request
Consultation & Support Free project consultation and technical support throughout the process.
Contact Contact us for a custom quote and to discuss your specific project requirements.
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