OLuc Luciferase (OLuc) EGFP mRNA

OLuc is an engineered luciferase derived from the 19 kDa catalytic subunit of the bioluminescent enzyme found in the deep-sea shrimp Oplophorus gracilirostris. It exhibits approximately 150-fold greater sensitivity compared to traditional firefly luciferase and utilizes furimazine as its substrate for bioluminescence detection.

This mRNA encodes a bicistronic construct comprising OLuc luciferase and EGFP, separated by a self-cleaving T2A peptide to enable independent expression of both proteins.

• 5' capped with ARCA (Anti-Reverse Cap Analog, m⁷(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m⁷G(5')ppp(5')(2'-OMeA)pG, Cap 1)
• Substituted with Pseudo-UTP
• Partially substituted with 5mCTP
• Poly(A) tail: 180 nucleotides