Human Klf4-Oct4-Sox2-Glis1-c-Myc (KOSGM) Self-Amplifying mRNA

Catalog #: EG-312
Size
10 µg
Catalog #: —
$298.00
Size
20 µg
Catalog #: —
$498.00
Size
100 µg
Catalog #: —
$2500.00
View Cart
Product Description
Product Name Human Klf4-Oct4-Sox2-Glis1-c-Myc (KOSGM) Self-Amplifying mRNA
Description

This self-amplifying mRNA encodes four nonstructural proteins (nsP1 to nsP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), and five human transcription factors (OCT4, KLF4, c-MYC, SOX2, GLIS1) linked by four 2A self-cleaving peptides
Applications Efficient, rapid, integration-free generation of pluripotent stem (iPS) cells from human fibroblasts.
Modifications
  • 5' Capped with vaccinia capping enzyme system and 2'-O-methyltransferase to yield cap-1 structure
  • Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Concentration ~1 µg/µl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC Agarose gel electrophoresis
Shipping Dry ice
Storage -80 oC
References Reprogramming human fibroblasts to pluripotency using modified mRNA. Mandal & Rossi, Nature Protocols 8,568-582 (2013) | doi:10.1038/nprot.nprot.2013.019.
Product Documents
DatasheetEG-312 Product Datasheet
Download PDF
Related Products
Human Klf4-Oct4-Sox2-c-Myc (KOSM) mRNA
Catalog #: EG-308$280.00
View Details
NLS-Cre Recombinase mRNA, Animal-free
Catalog #: EG-2895$480.00
View Details
NLS-FLP Recombinase mRNA, Animal-Free
Catalog #: EG-1044$480.00
View Details
NLS-FLP Recombinase Self-Amplifying mRNA, SA-NLS-FLP
Catalog #: EG-1045$980.00
View Details
Cy5-modified EGFP mRNA
Catalog #: EG-1046$560.00
View Details
Firefly Luciferase FLuc mRNA
Catalog #: EG-1047$280.00
View Details