NLS-eCas9 mRNA

Catalog #: EG-321
Size
ARCA 20 µg
Catalog #: —
$280.00
Size
ARCA 100 µg
Catalog #: —
$680.00
Size
ARCA 1 mg
Catalog #: —
$3680.00
Size
Cleancap 20 µg
Catalog #: —
$398.00
Size
CleanCap 100 µg
Catalog #: —
$680.00
Size
CleanCap 1 mg
Catalog #: —
$3680.00
Size
CleanCap 5 X 1 mg
Catalog #: —
$13180.00
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Product Description
Product Name NLS-eCas9 mRNA
Description

NLS-eCas9 is a mutant of Streptococcus pyogenes SF370 Cas9 protein with nuclear localization signal (NLS) at both N and C terminus. The mutant Cas9 reduces off-target effects by more than 10 folds.
Codon Optimization Human & mouse
Modifications
  • 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
  • Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Application CRISPR, Genome Enginering
Concentration ~1 μg/μl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC Agarose gel electrophoresis
Shipping Dry ice
Storage -80 oC
Product Documents
DatasheetEG-321 Product Datasheet
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