Anti-EGFRvIII Monoclonal Antibody, recombinant

• Rabbit monoclonal antibody produced in HEK293 cells under serum-free, protein-free conditions
• Purified by Protein A affinity chromatography
• High batch-to-batch consistency and reproducibility
• Long-term supply security

• Flow Cytometry / ELISA: 5 µg/mL final concentration
• Immunoprecipitation: 10 µg per 0.3–0.5 mg cell lysate
• Western Blot: 1 µg/mL final concentration

Fig. A: Flow cytometry assay demonstrating cell binding specificity. U87 cells (EGFRvIII negative), U87 (EGFR++), and U87 (EGFRvIII++) cells were stained with 5 µg antibody in 1 mL PBS containing 5% BSA. Binding was detected with a Cy5-conjugated goat anti-rabbit antibody. Shaded area: isotype control; red line: EGFRvIII antibody staining.
Fig. B: ELISA assay demonstrating target specificity. Recombinant EGFR-hFc (negative control), EGFRvIII-hFc (target), and BSA were immobilized at 5 µg/mL. Biotinylated antibody (1:2 serial dilutions from 200 nM) was incubated at 37 °C for 30 min. Binding was visualized with HRP-conjugated streptavidin.