Product Description
| Product Name | Anti-LAG3 Monoclonal Antibody, Recombinant |
| Catalog # | EG-374 |
| Size | 100 µg |
| Key Features |
|
| Clone Number | M36 |
| Immunogen | Recombinant extracellular domain of LAG3 expressed and purified from HEK293 cells |
| Target | Extracellular region of LAG3 (CD223) |
| Species Cross-Reactivity | Human |
| Applications | Flow Cytometry, Immunoprecipitation (IP), ELISA |
| Recommended Working Concentrations |
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| Background |
LAG3 (CD223) is an immune checkpoint receptor expressed on activated T cells. Its upregulation is critical for preventing T cell overactivation and autoimmunity. Persistent antigen stimulation leads to sustained LAG3 expression, contributing to T cell exhaustion. Neutralizing antibodies targeting LAG3, alone or combined with PD-1/PD-L1 blockade, are under clinical investigation as promising immunotherapies for solid tumors. Known LAG3 ligands include MHC class II, FGL-1, and Galectin-3. Additionally, LAG3 can be cleaved by ADAM10/17 metalloproteinases, releasing soluble forms into circulation. The M36 antibody weakly inhibits LAG3 binding to MHC II-expressing Raji cells at high concentrations. Reference: Lawrence P. Andrews, et al. LAG3 (CD223) as a cancer immunotherapy target. Immunol Rev. 2017;276:80-96. |
| Quality Control |
A) Flow cytometry analysis of LAG3 surface expression on activated peripheral blood mononuclear cells (PBMCs). PBMCs were stimulated in vitro with anti-CD3/anti-CD28 beads. 1 × 106 activated PBMCs were incubated with 5 µg antibody in 1 mL PBS containing 5% BSA. Binding was detected with Cy5-conjugated goat anti-rabbit secondary antibody. Shaded area: isotype control; red line: PBMCs activated for 1 day; green line: 3 days; blue line: 7 days. |
| Concentration | As indicated on the vial label |
| Formulation | 80% PBS, pH 7.4; 20% glycerol; 5% trehalose; 0.04% sodium azide (preservative) |
| Shipping | Shipped on blue ice |
| Storage |
-20 to -70 °C (long-term) 4 °C (up to one week) For frequent use, aliquot to avoid repeated freeze-thaw cycles. |
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