Thermus Aquaticus Single-stranded DNA-binding proteins

Catalog #: EG-1002
$495.00
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Product Description
Product Name Thermus aquaticus Single-Stranded DNA-Binding Protein (TaqSSB)
Overview The single-stranded DNA-binding protein from Thermus aquaticus (TaqSSB) is a thermostable DNA-binding protein essential for DNA replication, recombination, and repair in this thermophilic bacterium. Unlike most bacterial SSBs, which are tetramers, TaqSSB forms a functional dimer, with each monomer containing two oligonucleotide/oligosaccharide-binding (OB) domains. TaqSSB binds single-stranded DNA (ssDNA) with high specificity and affinity, protecting it from nucleolytic digestion and preventing secondary structure formation, thereby facilitating efficient DNA processing at elevated temperatures[1][2][3][4][5][6][7][8].
Size 100 µg
Concentration 1 mg/mL
Molecular Weight ~30 kDa (monomer); functional dimer ~60 kDa[6]
Key Features
  • Thermostable: active and stable at high temperatures (up to 90 °C)
  • Binds ssDNA with high affinity and specificity; does not bind well to double-stranded DNA[6]
  • Prevents formation of secondary structures and protects ssDNA from degradation
  • Unique dimeric structure, each monomer with two OB-folds[1][2][5][8]
  • Flexible C-terminal region critical for protein-protein interactions with DNA polymerase III and other partners[1][2][7]
Applications
  • Stabilization of ssDNA during high-temperature PCR and DNA amplification
  • In vitro DNA replication, repair, and recombination assays at elevated temperatures
  • Model system for studying thermostable DNA-protein interactions
Source Recombinant protein expressed in E. coli[6]
Quality Control
  • Purity >95% by SDS-PAGE
  • Confirmed single-stranded DNA binding activity[6]
Formulation 50 mM Tris-HCl (pH 8.0), 200 mM NaCl, 0.1 mM DTT, 0.5 mM EDTA, 50% glycerol[6]
Storage -20 °C; avoid repeated freeze-thaw cycles[6]
References
  • Fedorov, R., et al. 3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins. Nucleic Acids Res. 2006; 34(22):6708-6717.[1][2][5][7]
  • Yoshida, M., et al. Structure of the single-stranded DNA-binding protein SSB from Thermus aquaticus. Acta Cryst. 2006; F62: 1101-1104.[8]
  • UniProt Q9KH06: Thermus aquaticus SSB protein entry.[3]
  • AS ONE International. Thermus aquaticus Single-stranded DNA Binding Protein (SSB) Product Sheet.[6]
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