Deinococcus proteolyticus single-stranded DNA binding proteins (SSB)

Catalog #: EG-1005
$495.00
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Product Description
Product Name Deinococcus proteolyticus Single-Stranded DNA-Binding Protein (DprSSB)
Overview The single-stranded DNA-binding protein from Deinococcus proteolyticus (DprSSB) is a thermostable, homodimeric SSB essential for DNA replication, recombination, and repair in this extremophilic bacterium. Like other SSBs from the Deinococcus-Thermus group, DprSSB is structurally unique among bacterial SSBs: each monomer contains two oligonucleotide/oligosaccharide-binding (OB) folds, and the functional unit is a symmetric dimer. This arrangement results in a four-OB-fold DNA-binding surface, distinct from the homotetrameric, single-OB-fold-per-monomer SSBs found in most bacteria[1][2][5][6][8]. DprSSB binds single-stranded DNA with high affinity, protects it from degradation, and prevents secondary structure formation. It is also implicated in supporting RecA-mediated strand exchange and DNA repair, a hallmark of the robust genome maintenance systems in Deinococcus species.
Size 100 µg
Concentration 1 mg/mL
Molecular Weight ~31 kDa (monomer); ~62 kDa (homodimer functional unit)[8]
Key Features
  • Homodimeric structure, each monomer with two OB folds (four OB folds per dimer)[1][2][6][8]
  • Thermostable and active at elevated temperatures
  • High-affinity binding to single-stranded DNA; protects ssDNA and prevents secondary structure formation
  • Supports RecA-mediated DNA strand exchange and DNA repair pathways
  • Contributes to the extraordinary DNA damage resistance of Deinococcus species
Applications
  • Stabilization of ssDNA in high-temperature molecular biology assays
  • Studies of DNA replication, recombination, and repair in extremophiles
  • Model system for investigating unique SSB architectures and DNA-protein interactions
Source Recombinant protein expressed in E. coli
Quality Control
  • Purity >95% by SDS-PAGE
  • Functional validation in ssDNA binding assays
Formulation 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
Storage -80 °C (long-term); avoid repeated freeze-thaw cycles
References
  • Bernstein, D.A., et al. Crystal structure of the Deinococcus radiodurans single-stranded DNA-binding protein. Proc Natl Acad Sci USA. 2004; 101(7): 2273–2278.[1][2]
  • George, N.P., et al. Structure and cellular dynamics of Deinococcus radiodurans single-stranded DNA-binding protein-DNA complexes. J Biol Chem. 2012; 287(29): 24570–24582.[5][7]
  • Yamamoto, K., et al. Identification and properties of the Deinococcus grandis and Deinococcus proteolyticus single-stranded DNA binding proteins. J Biochem. 2007; 141(5): 653–661.[8]
  • Suzuki, S., et al. The essential role of the Deinococcus radiodurans ssb gene in cell survival after irradiation and oxidative stress. PLoS ONE. 2013; 8(8): e71651.[6]
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