3D Structure — Nanobody–Epitope Complex
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anti-GFP nanobody complex
PDB 6LR7
■ nanobody (VHH) ■ antigen ■ epitope (30)
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Interactive model of the VHH bound to its structural epitope on GFP (from experimental complex PDB 6LR7). Nanobody in blue, epitope contact residues in red. For research visualization.
Product Description
| Product Name | Anti-GFP Nanobody (VHH), Recombinant, Purified |
| Description | A purified, engineered single-domain antibody (VHH/nanobody) that binds GFP and its common derivatives with high specificity. Ideal for one-step immunoprecipitation of GFP-fusion proteins, IF, and live-cell imaging. |
| Target / Specificity | Green fluorescent protein (GFP) and derivatives (eGFP, YFP, CFP, Venus, Citrine, sfGFP) |
| Sequence | This nanobody is an engineered variant based on PDB 6LR7, with higher affinity and stability than the original published sequence. |
| Format | Purified recombinant single-domain antibody (VHH / nanobody), carrier-free |
| Tag & Expression | C-terminal 6×His tag; secreted from HEK293 cells and purified by one-step IMAC from the culture medium. |
| Molecular Weight | ~14 kDa (single VHH domain + His6; ~1/10 the size of a conventional IgG) |
| Purity | >90% by SDS-PAGE |
| Quantity | 100 µg |
| Formulation | PBS with 50% glycerol (carrier-free); 0.02% sodium azide |
| Applications | Immunoprecipitation (IP), pull-down of GFP fusions, immunofluorescence (IF), Western blot (WB), ELISA, live-cell imaging |
| Structural Epitope | Epitope mapped from the experimental VHH–antigen complex (PDB 6LR7); 30 antigen contact residues within 5 Å. An interactive 3D complex model is shown on this page. |
| Storage | Store at -20°C; avoid repeated freeze-thaw. Stable 1 year. |
| Advantages | Nanobodies are the smallest functional antibody fragments: exceptional thermal and chemical stability, single-domain, high-yield recombinant production, and access to cryptic epitopes and dense tissue that conventional antibodies cannot reach. |
Product Documents
DatasheetEG-NB-GFP01 Product Datasheet
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