Anti-Green fluorescent protein Nanobody (VHH), Recombinant, Purified
Catalog #: EG-NB-7E53
$355.00
3D Structure — Nanobody–Epitope Complex
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anti-Green fluorescent protein nanobody complex
PDB 7E53
■ nanobody (VHH) ■ antigen ■ epitope (29)
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Interactive model of the VHH bound to its structural epitope on Green fluorescent protein (from experimental complex PDB 7E53). Nanobody in blue, epitope contact residues in red. For research visualization.
Product Description
| Product Name | Anti-Green fluorescent protein Nanobody (VHH), Recombinant, Purified |
| Description | A purified, engineered single-domain antibody (VHH / nanobody) against Green fluorescent protein, for research applications including detection, capture and imaging. |
| Target / Specificity | Green fluorescent protein |
| Sequence | This nanobody is an engineered variant based on PDB 7E53, with higher affinity and stability than the original published sequence. |
| Format | Purified recombinant single-domain antibody (VHH / nanobody), carrier-free |
| Tag & Expression | C-terminal 6×His tag; secreted from HEK293 cells and purified by one-step IMAC from the culture medium. |
| Molecular Weight | ~14 kDa (single VHH domain + His6; ~1/10 the size of a conventional IgG) |
| Purity | >90% by SDS-PAGE |
| Quantity | 100 µg |
| Formulation | PBS with 50% glycerol (carrier-free); 0.02% sodium azide |
| Applications | ELISA, Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), affinity capture |
| Structural Epitope | Epitope mapped from the experimental VHH–antigen complex (PDB 7E53); 29 antigen contact residues within 5 Å. An interactive 3D complex model is shown on this page. |
| Storage | Store at -20°C; avoid repeated freeze-thaw. Stable 1 year. |
| Advantages | Nanobodies are the smallest functional antibody fragments: exceptional thermal and chemical stability, single-domain, high-yield recombinant production, and access to cryptic epitopes and dense tissue that conventional antibodies cannot reach. |
Product Documents
DatasheetEG-NB-7E53 Product Datasheet
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