3D Structure — Nanobody–Epitope Complex
Loading 3D structure…
anti-Pyruvate kinase nanobody complex
PDB 8RTF
■ nanobody (VHH) ■ antigen ■ epitope (18)
Drag to rotate · scroll to zoom
Interactive model of the VHH bound to its structural epitope on Pyruvate kinase (from experimental complex PDB 8RTF). Nanobody in blue, epitope contact residues in red. For research visualization.
Product Description
| Product Name | Anti-Pyruvate kinase Nanobody (VHH), Recombinant, Purified |
| Description | A purified, engineered single-domain antibody (VHH / nanobody) against Pyruvate kinase, for research applications including detection, capture and imaging. |
| Target / Specificity | Pyruvate kinase |
| Sequence | This nanobody is an engineered variant based on PDB 8RTF, with higher affinity and stability than the original published sequence. |
| Format | Purified recombinant single-domain antibody (VHH / nanobody), carrier-free |
| Tag & Expression | C-terminal 6×His tag; secreted from HEK293 cells and purified by one-step IMAC from the culture medium. |
| Molecular Weight | ~14 kDa (single VHH domain + His6; ~1/10 the size of a conventional IgG) |
| Purity | >90% by SDS-PAGE |
| Quantity | 100 µg |
| Formulation | PBS with 50% glycerol (carrier-free); 0.02% sodium azide |
| Applications | ELISA, Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), affinity capture |
| Structural Epitope | Epitope mapped from the experimental VHH–antigen complex (PDB 8RTF); 18 antigen contact residues within 5 Å. An interactive 3D complex model is shown on this page. |
| Storage | Store at -20°C; avoid repeated freeze-thaw. Stable 1 year. |
| Advantages | Nanobodies are the smallest functional antibody fragments: exceptional thermal and chemical stability, single-domain, high-yield recombinant production, and access to cryptic epitopes and dense tissue that conventional antibodies cannot reach. |
Product Documents
DatasheetEG-NB-8RTF Product Datasheet
Download PDFRelated Products
Anti-GFP Nanobody (VHH), Recombinant, Purified
Catalog #: EG-NB-GFP01$355.00
Anti-RFP / mCherry Nanobody (VHH), Recombinant, Purified
Catalog #: EG-NB-RFP01$355.00
Anti-EGFR Nanobody (VHH), Recombinant, Purified
Catalog #: EG-NB-EGFR01$425.00
Anti-PD-L1 Nanobody (VHH), Recombinant, Purified
Catalog #: EG-NB-PDL101$425.00
Anti-SARS-CoV-2 Spike Nanobody (VHH), Recombinant, Purified
Catalog #: EG-NB-SPK01$425.00
Anti-Influenza Hemagglutinin (HA) Nanobody (VHH), Recombinant, Purified
Catalog #: EG-NB-HA01$445.00